goat anti mouse igs conjugated to allophycocyanin  (SouthernBiotech)

Journal: bioRxiv

Article Title: Lung endothelial niche signaling governs self-renewal and fate transitions of human alveolar stem cells

doi: 10.64898/2026.03.07.710316

Figure Lengend Snippet: a. Representative images showing the selection process to enrich AT2 cell purity. Scale bar = 300 µm. b. Immunofluorescence staining of SP-C in unsorted lung cells and cells sorted using collagen I and HT2-280 antibody. Scale bar = 100 µm. The graph shows the percentage of SP-C⁺ cells (n = 3). c. Phase-contrast images after co-culture of indicated cells with AT2 cells. Scale bar = 200 µm. The graph shows the number of recovered cells (n = 3). d. Immunofluorescence images of SP-C, AGER and KRT5 in co-cultured cells and recovered cells. The graph shows the percentage of cells expressing each marker protein (n = 9). e. Phase images of adult lung fibroblast (ALF) and lung endothelial cell (LEC) and growth factor array blots using ALF- or LEC-conditioned medium. Scale bar = 250 µm. Quantification of indicated growth factors from ALF and LECs are shown on the right. Protein expression levels are presented as relative values, normalized to the ALF group. f–g. AT2 cell counts after culture with the indicated growth factors (n = 3). Statistical significance was determined by Student’s t test, and data are represented as mean ± SEM.

Article Snippet: Cells were pelleted (500 g, 5 min), resuspended in 500 μL PBS-S (PBS containing 1% FBS), and incubated with 25 μL anti-human HT2-280 mouse IgM (Terracebiotech, TB-27AHT2-280) for 15 min on ice.

Techniques: Selection, Immunofluorescence, Staining, Co-Culture Assay, Cell Culture, Expressing, Marker